variant ii hplc system Search Results


rp hplc  (Agilent technologies)
95
Agilent technologies rp hplc
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Rp Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high performance liquid chromatography  (Bio-Rad)
96
Bio-Rad high performance liquid chromatography
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
High Performance Liquid Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rp-hplc gilson®, 30×100  (Gilson Inc)
90
Gilson Inc rp-hplc gilson®, 30×100
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Rp Hplc Gilson®, 30×100, supplied by Gilson Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoassay ion exchange high performance liquid chromatography hplc system  (Bio-Rad)
96
Bio-Rad immunoassay ion exchange high performance liquid chromatography hplc system
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Immunoassay Ion Exchange High Performance Liquid Chromatography Hplc System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quality control  (Sysmex Corporation)
86
Sysmex Corporation quality control
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Quality Control, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high performance liquid chromatography hplc variant ii analyzer  (Bio-Rad)
95
Bio-Rad high performance liquid chromatography hplc variant ii analyzer
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
High Performance Liquid Chromatography Hplc Variant Ii Analyzer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hplc  (Varian Medical)
86
Varian Medical hplc
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Hplc, supplied by Varian Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hplc variant ii  (Bio-Rad)
93
Bio-Rad hplc variant ii
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Hplc Variant Ii, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dca vantage  (Siemens AG)
90
Siemens AG dca vantage
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Dca Vantage, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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preparative hplc  (Varian Medical)
86
Varian Medical preparative hplc
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Preparative Hplc, supplied by Varian Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad variant ii hplc thalassemia assay  (Bio-Rad)
93
Bio-Rad bio rad variant ii hplc thalassemia assay
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Bio Rad Variant Ii Hplc Thalassemia Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prep hplc  (Varian Medical)
86
Varian Medical prep hplc
( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed <t>by</t> <t>RP-HPLC</t> and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion
Prep Hplc, supplied by Varian Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed by RP-HPLC and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion

Journal: bioRxiv

Article Title: Mechanism of the secretion of the lanthipeptide nisin

doi: 10.1101/839423

Figure Lengend Snippet: ( A ) The supernatants of pre-NisA secreting L. lactis NZ9000 strains was analyzed by RP-HPLC and the amount of pre-NisA was determined. Amounts of secreted pre-peptides (nmol) are plotted against time (min) and the resulting curves were fitted by an allosteric sigmoidal fit. Modified NisA (mNisA, red) was secreted by strain NZ9000BTC (red dots) and can be preclude by nisT deletion (strain NZ9000BC, clear dot) or an ATP-deficient mutant (NZ9000BT H551A C, red rhomb). Dehydrated NisA (dNisA, blue) was secreted by strains NZ9000BTC H331A (blue rhomb) and NZ9000BT (blue dot), whereas unmodified NisA (uNisA, grey) was secreted by the strains NZ9000T (grey dots) and NZ9000TC (grey square). Dashed square shows a zoom-in on strains with lower secretion level. ( B ) The kinetic parameter of V max (nmol) of secreted peptides was plotted as bars against the various secretion systems. ( C ) The secretion rate of NisA molecules per NisT molecules was plotted against time (min) and fitted by linear regression. The slope represented the secretion rate of NisA•NisT −1 •min −1 for the strains NZ9000BTC and NZ9000T. All data represent secretion experiments from at least five different transformants and are represented as means ± SD (n=5). ++: WT secretion; o: low secretion; −: no secretion

Article Snippet: The culture supernatants containing pre-NisA variants were analyzed by RP-HPLC (Agilent Technologies 1260 Infinity II).

Techniques: Modification, Mutagenesis

The supernatants of pre-NisA secreting L. lactis NZ9000 strains were employed for an RP-HPLC analysis. The pre-NisA variants (mNisA, dNisA and uNisA) were separated from other peptides in the supernatant by an acetonitrile/water gradient on a C-18 RP-HPLC column (left panel). The elution fractions (dashed square; middle panel) were further analyzed by MALDI-TOF-MS (right panel) to verify the correct mass. Integration of the corresponding peaks enables the determination of peptide amounts (nmol). Supernatant analysis of L. lactis strains ( A ) NZ9000BTC, ( B ) NZ9000BTC H331A , ( C ) NZ9000BT H551A C, ( D ) NZ9000BT, ( E ) NZ9000T, ( F ) NZ9000TC and ( G ) NZ9000BC.

Journal: bioRxiv

Article Title: Mechanism of the secretion of the lanthipeptide nisin

doi: 10.1101/839423

Figure Lengend Snippet: The supernatants of pre-NisA secreting L. lactis NZ9000 strains were employed for an RP-HPLC analysis. The pre-NisA variants (mNisA, dNisA and uNisA) were separated from other peptides in the supernatant by an acetonitrile/water gradient on a C-18 RP-HPLC column (left panel). The elution fractions (dashed square; middle panel) were further analyzed by MALDI-TOF-MS (right panel) to verify the correct mass. Integration of the corresponding peaks enables the determination of peptide amounts (nmol). Supernatant analysis of L. lactis strains ( A ) NZ9000BTC, ( B ) NZ9000BTC H331A , ( C ) NZ9000BT H551A C, ( D ) NZ9000BT, ( E ) NZ9000T, ( F ) NZ9000TC and ( G ) NZ9000BC.

Article Snippet: The culture supernatants containing pre-NisA variants were analyzed by RP-HPLC (Agilent Technologies 1260 Infinity II).

Techniques: